differential interference contrast
- differential interference contrast
-
Method of image formation in the light microscope based on the method proposed by Nomarski (though strictly speaking all forms of optical microscopy rely to
a greater or lesser extent on differential interference). The light beam is split by
a Wollaston prism in the condenser, to form slightly divergent beams polarized at right angles. One passes through the specimen (and is retarded if the refractive index is greater), and one through the background nearby: the two are recombined in
a second
Wollaston prism in the objective and interfere to form an image. The image is spuriously "three-dimensional" - the nucleus, for example, appears to stand out above the
cell (or be hollowed out) because it has
a higher refractive index than the cytoplasm. The Nomarski system has the advantage that there is no phase-halo, but the contrast is low and image formation with crowded cells is poor because the background does not differ from the specimen.
Dictionary of molecular biology.
2004.
Look at other dictionaries:
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